Keywords
cell culture
glial cells
spinal ganglia
cryoextract
reproduction
neurotrophins
How to Cite
Abstract
In the context of a growing global trend toward delayed motherhood, which is often accompanied by age-related decline in ovarian reserve and impaired reproductive function, research aimed at developing novel strategies for the assisted reproductive technologies is gaining particular significance. One of the promising approaches is the use of cell-based products in particular biologically active compositions that have a neurotrophin-like effect on the female reproductive system.
This study investigates the effects of biologically active compositions—including cryoextracts and conditioned media of cells isolated from the spinal ganglia of neonatal piglets—on the functional state of the reproductive system in female rats at a late reproductive age. Specifically, the study focuses on changes in the parameters of the estrous cycle following administration of these compositions.
The results revealed a statistically significant prolongation of the estrus phase in treated animals compared to intact controls, indicating potential activation of ovarian function and a favorable modification of reproductive status. These findings suggest that neurotrophic factors contained in the tested biological products may influence the regulation of primordial follicle development and maintenance of the ovarian reserve. This opens new prospects for the development of therapeutic agents aimed at fertility restoration, particularly in women with diminished ovarian reserve or poor response to conventional gonadotropin stimulation protocols in assisted reproductive technology programs.
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Copyright (c) 2025 Galyna Bozhok, Nataliia Komaromi, Viktoriia Ustychenko, Olena Protsenko, Nataliia Remnyova, Yevgen Legach (Author)